mpm cell lines h2052  (ATCC)

Journal: Translational Cancer Research

Article Title: Induction of metallothionein expression by supplementation of zinc induces resistance against platinum-based treatment in malignant pleural mesothelioma

doi: 10.21037/tcr-22-2651

Figure Lengend Snippet: Gene expression of MT2A after supplementation of different zinc concentrations. The data were measured 77 h after supplementation of zinc. The difference in MT2A expression of the sample between zinc-treatment and control is shown on the y-axis (2 −ΔCp ) (Cp means the crossing point describing the cycle at which the fluorescence first rises significantly above the background fluorescence). All MPM cell lines showed elevated expression of MT2A by adding higher concentrations of zinc [(A) MSTO211H (P=0.0833), (B) NCI-H2452 (P=1), and (C) NCI-H2052 (P=0.0833)]. P values were calculated by using the Spearman’s rank correlation test. MT2A , metallothionein IIA; MPM, malignant pleural mesothelioma.

Article Snippet: All MPM cell lines [NCI-H2052: CRL-5915; NCI-H2452: CRL-5946; MSTO-211H: CRL-2081; American Type Culture Collection (ATCC), Virginia, USA] were cultured in Roswell Park Memorial Institute 1640 medium (RPMI) (Thermo Fisher Scientific, Massachusetts, USA), supplemented with 10% fetal calf serum (FCS) and 1% penicillin and streptomycin (P/S, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Gene Expression, Expressing, Control, Fluorescence

Journal: Oncotarget

Article Title: Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

doi: 10.18632/oncotarget.28134

Figure Lengend Snippet: Spheroids generated with 1000 cells per spheroid and cultured for 13 days in complete RPMI medium at 37°C and 5% CO 2 showed different shapes and sizes. Representative images of H2052, H2052/484, H2452, LuCa1, LuCa61, and LuCa62 spheroids viewed from above by inverted phase contrast microscopy. Scale bar represents 100 μm.

Article Snippet: The human MPM cell lines H2052 (NCI-H2052), and H2452 (NCI-H2452) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Generated, Cell Culture, Microscopy

Journal: Oncotarget

Article Title: Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

doi: 10.18632/oncotarget.28134

Figure Lengend Snippet: ( A ) Representative images and ( B ) spheroid diameters of the MPM H2052/484 (left) and the lung adenocarcinoma LuCa1 (right) spheroids grown at increasing seeding densities (from 10 3 to 10 5 cells/spheroid) between days 1 and 3. Scale bar represents 100 μm.

Article Snippet: The human MPM cell lines H2052 (NCI-H2052), and H2452 (NCI-H2452) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Oncotarget

Article Title: Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

doi: 10.18632/oncotarget.28134

Figure Lengend Snippet: ( A ) Representative images of the luminescent signals of 3 H2052/484 spheroids grown at the seeding densities of 750 cells/spheroid at day 3 before and after sonication. ( B ) Ratio of ATPi levels measured before and after sonication of H2052/484, H2052, H2452, LuCa1, LuCa61, and LuCa62 spheroids after seeding 750 cells/spheroid. The data represent the mean ± SEM of 3 independent experiments. The ATPi levels measured after sonication were increased compared with those before sonication and statistically significant for LuCa1 and LuCa62 spheroids ( ** P < 0.01). ( C ) ATPi levels of H2052/484 and LuCa1 spheroids at day 3 post-seeding of 10 3 – 10 5 cells/spheroid. The ATPi levels increased with cell number per spheroid and were significantly different between 10 3 cells/spheroid and 10 5 cells/spheroid ( * P < 0.05). The data represent the mean ± SD of 2 independent experiments for each cell density. ( D ) Correlation of the ATPi levels and the spheroid size of H2052/484 and LuCa1 spheroids at day 3 post-seeding of 750 cells/spheroid. Kinetics of ATPi of MPM ( E ) and lung adenocarcinoma ( F ) spheroids over 27 days at the optimal seeding densities (1000 cells/spheroid). The data represent the mean ± SD of 3 independent experiments.

Article Snippet: The human MPM cell lines H2052 (NCI-H2052), and H2452 (NCI-H2452) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Sonication

Journal: Oncotarget

Article Title: Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

doi: 10.18632/oncotarget.28134

Figure Lengend Snippet: At day 3 post-seeding, H2052/484 spheroids (1000 cells/spheroid) were treated for 3 hours with different concentrations of cisplatin (50, 100, or 200 μM) ( A , B ) or cisplatin/pemetrexed combination (50/200, 100/400, or 200/800 μM) ( C , D ). After washes, the spheroids were cultured for 17 days, the growth (A, C) and the ATPi kinetics (B, D) were assessed. The data represent the mean ± SD of 3 independent experiments. * P < 0.05; ** P < 0.01 comparing treated groups with control group.

Article Snippet: The human MPM cell lines H2052 (NCI-H2052), and H2452 (NCI-H2452) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Cell Culture, Control

Journal: Oncotarget

Article Title: Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

doi: 10.18632/oncotarget.28134

Figure Lengend Snippet: H2052/484 spheroids (1,000 cells/spheroid) were untreated (control, black curve) or treated with compound for 3 hours at day 3 post-seeding. After washes, the spheroids were cultured for 24 hours and the ATPi levels were determined. Data are presented as the ratio of the ATPi levels of treated spheroid to control spheroid. The data represent the mean ± SD of 2 independent experiments.

Article Snippet: The human MPM cell lines H2052 (NCI-H2052), and H2452 (NCI-H2452) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Control, Cell Culture